FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l06_n02_B3W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l06_n02_B3W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28620659
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT28950.012.6792232
GTACTCG63050.010.3924174
TACTCGA69200.09.6959225
GCGTGAC41600.09.5269511
AGTACTC74550.09.182783
GGAAAAT106300.09.0666131
GTAGAAC39400.08.95875911
GTGACTC45400.08.531413
AATCGAA51800.08.4323955
ACACGCG27300.08.3209036
GAATCGA44100.08.2204564
CTCTATA65100.07.784277445
AAAATCC73800.07.68939973
AGAACGG182750.07.5658763
TCACACG31200.07.497182415
GCCGAAT21600.07.4018311
GGTAGGC27400.07.38527777
CACTCGT67150.07.369677535
AAAAATC71550.07.3333072
GAAAAAT146900.07.1739541
Produced by FastQC (version 0.11.5)