FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l06_n02_B3W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l06_n02_B3W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29529926
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT31100.014.0473332
GCGTGAC44500.010.5760821
GTACTCG56100.010.3953184
GTAGAAC40600.010.244369511
GTGACTC48650.09.3036313
GGAAAAT127500.08.8307651
TACTCGA65300.08.7921575
AATTCAC48550.08.6256565
AATTGAC46800.08.4671125
TCAGGTA57800.08.402087
AGTACTC73450.08.277733
CTCTATA68700.08.06465145
GAATTGA68650.08.035754
AATCGAA56700.07.9417355
CACTCGT70250.07.813004535
GGTAGAA55750.07.70243410
TCACACG28700.07.680040415
GGGAAAT64950.07.66216751
GCTCTAG59650.07.65112111
AAAATCC94200.07.60178383
Produced by FastQC (version 0.11.5)