FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l06_n02_B3W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l06_n02_B3W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26031877
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT20050.012.2424512
GTAGAAC47650.011.65348511
TCAGGTA59100.010.0431527
TAGAACA60100.09.27676412
GGAAAAT122500.08.8602561
GGTAGAA66600.08.77649210
CAGGTAG68350.08.6848
AATCGAA52900.08.5971235
GCGTGAC31250.08.5749631
AGGTAGA70200.08.2942949
GTACTCG51500.08.13205054
GGGGAAT51700.07.75291061
GGAAGAT176900.07.58671431
GAATCGA45500.07.5713684
AAAATCC87650.07.55329753
GAATTGA62650.07.4754364
AATTGAC40100.07.4111755
GGGAAAT59250.07.41108131
GGAGAAT79800.07.3367851
AGATCAG105250.07.27362874
Produced by FastQC (version 0.11.5)