FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l06_n02_B3W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l06_n02_B3W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29885923
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT29250.015.9367792
GCGTGAC43100.012.0689741
GTACTCG70950.010.8216964
AGTACTC78500.010.2121913
TACTCGA75950.010.1085615
GTGACTC48250.010.034133
GTAGAAC45350.09.5677311
GGAAAAT131050.09.3647271
AAAATCC91100.08.5772823
AATCGAA52900.08.5546675
TCAGGTA59400.08.1755297
GAATTGA70150.08.056374
GAATCGA42600.08.0339164
TGACTCT62900.08.01845554
GCTCTAG60750.07.845460411
CTCGAAT23250.07.84508561
GGGAAAT63550.07.7246111
AATTGAC48450.07.7139545
AGTGTGA65250.07.548234514
CACTCGT61700.07.47385135
Produced by FastQC (version 0.11.5)