FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l06_n02_B3W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l06_n02_B3W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26143513
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT27600.015.99182
GCGTGAC39750.012.3495091
GTGACTC40250.011.9167163
GTACTCG52400.010.6996014
AGTACTC58750.09.8890523
TACTCGA56550.09.8340835
GCTCTAG53400.09.2622111
GGAAAAT108250.09.0488151
AATCGAA43450.08.860865
GAATCGA38600.08.8082414
TGACTCT56900.08.6266814
GAATTGA63200.08.5149154
ACTCTGC66100.08.1320236
TCACACG26850.07.957878615
AAAATCC79550.07.95442873
CTCTATA51850.07.948958445
AATTGAC42050.07.65666345
GTAGAAC33150.07.460060611
CCAGTGT54400.07.356114412
AAATTGA74150.07.25748734
Produced by FastQC (version 0.11.5)