FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l06_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l06_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13945025
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG4450.034.86187715
CGTCTGA4750.032.1874316
CACACGT5250.029.546212
CACGTCT5900.026.29196214
ACACGTC6100.025.42910613
CAGTCAC5200.023.77794627
GCACACG7250.021.70560511
TCGGAAG10400.021.4623323
ATCGGAA10700.020.8597562
AGCACAC7550.020.24761210
CCAGTCA6600.020.0966226
CGGAAGA11750.018.6107374
GATCGGA12200.018.1103631
CTACGCT750.001116425817.98709520
CCGAGAC4000.017.43031129
GAGACCG4100.017.01635231
CTCCAGT8650.016.37330424
AGTCAGT1301.087113E-515.6084611
ACCGATC4600.014.68118734
CGAGACC5300.014.00853330
Produced by FastQC (version 0.11.5)