FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l06_n01_B3W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l06_n01_B3W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25387828
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATC40450.028.72586438
ACGTCTG43100.028.64766115
CGTCTGA46850.026.21123116
CCGTACG47350.025.10696433
TCGTATG40550.024.54935845
CTCGTAT44700.023.22748444
ACCGTAC54800.023.00683632
CACCGTA53650.022.61504231
CACGTCT61200.020.7616614
ATCGGAA59700.020.6496352
TCGGAAG61550.020.211723
CACACGT65850.018.95327612
GTAATCT63050.018.82530239
TACGTAA64250.018.57362736
CAGTCAC65800.018.28415727
ACGTAAT65400.017.86887737
AATCTCG66050.017.32206341
ACACGTC79550.016.31107913
AGTCACC75700.016.2201228
TAATCTC75550.015.769937540
Produced by FastQC (version 0.11.5)