FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l06_n01_B3W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l06_n01_B3W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25736957
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG38500.033.62732333
AGTCACG46900.027.90664928
ACGTCTG49650.027.13290815
GTTTCGG49000.026.60620134
TTTCGGA49700.025.77841835
TTCGGAA51450.024.98956536
CGTCTGA55350.024.50187916
ACGTTTC54700.023.8310232
GTCACGT57150.022.79210529
TCGGAAT59200.021.68093737
ATCGGAA61600.021.655752
CTCGTAT42200.021.5079744
TCGGAAG64400.020.8888533
TCGTATG41400.020.01931645
CGGAATC68700.018.78263738
CACGTTT73850.018.10602831
CACGTCT75950.017.97319214
ACACGTC77400.017.95532213
CACACGT78500.017.18807812
CAGTCAC82850.016.7199127
Produced by FastQC (version 0.11.5)