FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l06_n01_B3W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l06_n01_B3W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29959625
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG51050.025.85749628
ACGTCTG53700.025.21122215
CACGTGG54850.024.09186731
CGTCTGA56400.023.72597316
TCGTATG46950.021.63528445
GTCACGT62450.021.11018429
CTCGTAT50550.020.76303344
GGCCTTA65850.020.14113236
GCCTTAT65250.019.87834237
TCGGAAG70550.019.4179923
ACGTGGC69000.019.0891632
CACGTCT72750.018.73223314
ATCGGAA72700.018.7199632
CTTATCT77150.016.87427739
CCTTATC79550.016.39103138
CACACGT85600.016.26129712
TATCTCG72800.016.24253841
CAGTCAC85850.015.82103827
ACACGTC93850.015.23916513
TCACGTG89000.014.9443630
Produced by FastQC (version 0.11.5)