FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l06_n01_B3W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l06_n01_B3W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25865151
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG30200.019.09074245
AGTCACG30400.018.41978628
ACGTCTG33650.017.4439715
CGTGAAA34050.016.46500633
CGTCTGA35600.016.29945416
CTCGTAT34300.015.36431544
ACGTGAA40450.014.58242232
AACGATC36400.014.47606438
CACGTGA42900.013.69534431
GAAACGA44000.013.253921536
TGAAACG46950.012.56489335
TCGGAAG47900.012.5396593
GTCACGT45450.012.52336229
AAACGAT47250.012.15210837
ATCGGAA49450.012.0101272
CACGTCT53350.011.63409614
TATAGGC48800.011.1979527
TCACGTG52950.010.96520130
CACACGT59350.010.57147512
CAGTCAC60150.09.87007327
Produced by FastQC (version 0.11.5)