FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l06_n01_B3W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l06_n01_B3W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28620659
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG41000.023.3104228
ACGTCTG42050.023.26476315
CGTCTGA44250.022.26095416
TCGTATG40850.020.89641245
ACGTCCG47100.020.65120132
CTCGTAT43750.019.92333444
GTCCGCA50450.019.058334
GTCACGT55750.017.55378529
ATCGGAA57550.017.0419962
TCGGAAG58450.016.9720133
CCGCACA57950.016.43662636
CACGTCT63400.015.99702114
TCACGTC64800.015.03777230
CACACGT69700.014.42163312
CGCACAT67100.013.9943137
CAGTCAC70300.013.97866827
ACACGTC82100.012.84604213
TCCGCAC80000.012.66601335
CGGAAGA89650.011.5170624
CGTCCGC88700.011.37271733
Produced by FastQC (version 0.11.5)