FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l06_n01_B3W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l06_n01_B3W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29529926
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG45250.021.81891415
CGTCTGA45500.021.60068716
GTCCGCA46100.020.75759536
TCGTATG39650.019.4259945
CTCGTAT41000.019.06116944
ATCGGAA59250.016.6299672
TCGGAAG61350.016.20743
CACGTCT67450.014.936975514
TCCGCAT65750.014.38345437
CACACGT70150.014.2974912
ACCCGTC69350.014.15418632
CGCATCT63350.014.149539
CAGTCAC73050.013.85303527
TCACCCG75700.012.93196630
CCGTCCG84650.012.47473234
AGTCACC85350.012.30482928
ACACGTC83100.012.23179213
CCCGTCC82800.011.85580833
CGTCCGC91600.011.626554535
GATCGGA92350.010.8400051
Produced by FastQC (version 0.11.5)