FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l06_n01_B3W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l06_n01_B3W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26031877
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG339070.13025184469026188TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG112300.034.52549715
CGTCTGA116350.033.30527516
ATCGGAA128450.030.4207532
CACACGT131950.029.65432712
TCGGAAG133000.029.5153563
CACGTCT134900.029.10653714
CTCGTAT62100.028.977844
GTCACAT131850.028.95482629
CAGTCAC132200.028.8838127
TCGTATG55950.028.8217745
GTCAGAA131250.028.51270136
TGTCAGA133400.028.28881835
AGTCACA134600.028.25195328
ACACGTC148350.026.58828213
ACATGTC149400.025.57316432
TCACATG151000.025.27566530
ATGTCAG152950.024.92316834
AATCTCG105850.024.91588241
GATCGGA158750.024.912241
CACATGT154750.024.568631
Produced by FastQC (version 0.11.5)