FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l06_n01_B3W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l06_n01_B3W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29885923
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTCCG42150.026.49533734
TCCGTAT42350.026.47769537
ACGTCTG44700.025.50870715
CGTATCT43350.025.24948539
CCGTATC43700.025.14650238
CGTCTGA47650.023.93006916
TTCCGTA49700.022.6066436
TCGTATG42200.022.0944845
CTCGTAT45300.021.1793444
GTTCCGT57400.019.92684735
TCGGAAG61950.018.8831443
ATCGGAA65050.017.7411752
CACGTCT68550.017.15769614
GTCACAG69100.016.53923429
CACACGT71950.016.25270712
TATCTCG66750.015.48682741
CAGTCAC79750.015.02967727
TCACAGT78600.014.65897630
ACAGTTC78550.014.5027532
CACAGTT80350.014.31591931
Produced by FastQC (version 0.11.5)