FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l06_n01_B3W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l06_n01_B3W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26143513
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG49700.028.01009815
CGTCTGA51550.027.0490816
TCGTATG44500.025.9642745
CTCGTAT47450.024.96730244
TCGGAAG63050.022.406443
ATCGGAA65950.021.2506182
CACAGTC67650.020.63081731
CACGTCT69100.020.53533414
GTCACAG72300.019.3228329
CAGTCAC74250.019.29231327
CACACGT74250.019.23173912
AGTCACA75950.018.56458528
AGTCAAC78750.018.04185334
TCACAGT78000.017.94542330
AATCTCG73450.017.81584541
ACACGTC87500.016.62790513
ACAGTCA86500.016.6059832
GTCAACA88700.015.96722135
TCTCGTA80250.015.2958543
CCAGTCA92750.015.27453226
Produced by FastQC (version 0.11.5)