FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l05_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l05_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13982952
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN772810.552680149370462No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTT6400.028.455992
TAGATCT3800.027.7758133
TCTCGGT3800.027.324237
AGTGTAG4100.025.19769729
GAGTGTA4150.024.3550428
GTAGATC4250.024.30600432
GTGTAGA4550.023.19729830
GTGTAGG4850.020.85564417
TGTAGAT5000.020.6592131
TCGTGTA5050.020.00805515
GTAGGGA4950.019.99268519
TGTAGGG5250.019.70529718
GATCTCG5700.019.31059635
TTCCCTA4000.019.07888
TAGGGAA5350.018.91940120
TCCCTAC3750.017.9558079
CTCGGTG6500.017.74706838
AGATCTC6100.017.67149434
TCGGTGG6400.016.6138839
CGGTTCA2950.015.98061512
Produced by FastQC (version 0.11.5)