FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l05_n02_E3W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l05_n02_E3W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24913200
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT29800.013.67922
GCGTGAC37550.011.9952371
GTAGAAC32200.010.47216911
GTACTCG54400.010.4319394
GTGACTC44400.09.9918883
AGTACTC60350.09.776613
TACTCGA56900.09.6561945
GGAAAAT105650.09.5285491
AAAATCC77250.09.3285863
AATTGAC40150.08.973525
GAATTGA58750.08.6629134
GCTCTAG53900.08.42488311
GAATCGA40550.08.219294
TGACTCT57400.08.1212314
TCACACG25100.07.974245515
AATCGAA44150.07.8034955
TAGAACA42400.07.795179412
CTCTATA52350.07.78740345
AAATCCT82300.07.68900064
GAAAATC88350.07.62189632
Produced by FastQC (version 0.11.5)