FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l05_n02_E3W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l05_n02_E3W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26662323
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCGA38800.012.3627434
GTAGAAC54200.012.23592211
CTAATCG34800.011.84246353
AATCGAG57950.010.6470415
TAGAACA63150.010.61028512
TCAGGTA66750.010.5764047
CAGGTAG70600.09.8077218
GGTAGAA69450.09.74298110
AATCGAA48100.09.1289615
GGAAAAT141450.09.059481
GCTAATC56650.08.746362
AGATCAG114000.08.6919014
AGGTAGA80200.08.6053749
AAAATCC97550.08.4493653
GAATCGA41900.08.4382534
GGGGAAT51600.08.3363821
GTACTCG39000.08.0263334
AGCATAA110950.08.016815
CATAAGC116950.07.88212447
AATTGAC42400.07.8069845
Produced by FastQC (version 0.11.5)