FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l05_n02_E3W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l05_n02_E3W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27422074
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT22600.011.0615382
GTAGAAC38500.010.56868111
GTACTCG42250.09.061124
AATCGAA51150.08.4964435
GGAAAAT107050.08.3732221
TCAGGTA55050.08.0047487
GCGTGAC35000.07.91467861
AATTGAC42450.07.9037655
CTCTATA58750.07.82108545
TAGAACA51750.07.82067812
GAATTGA67100.07.7861924
CACTCGT57550.07.777443435
AAAATCC77250.07.66697553
ATCTAGC47250.07.66369256
TACTCGA50050.07.6483955
GGTAGAA54250.07.624445410
ACTCGTG59200.07.600875436
GCGGAAA68800.07.594441
CAGGTAG57350.07.3303218
AAAAATC77200.07.26412062
Produced by FastQC (version 0.11.5)