FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l05_n02_E3W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l05_n02_E3W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23566756
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN325660.13818618056723633No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT17150.011.95033552
GTAGAAC36350.010.14256111
AATCGAA37700.09.5566085
GAATCGA33850.09.3805664
GTACTCG43250.09.320424
GGAAAAT94200.09.3004171
AAAATCC72200.08.7959043
TCACACG22550.08.77618715
GCGTGAC27450.08.5328621
AGAACGG150100.07.9817763
CACGTAA30150.07.832815616
TCAGGTA51100.07.8316537
GAATTGA56400.07.82610374
GGTAGAA46850.07.82129710
AGTACTC50750.07.7655213
GGTAGGC20850.07.76391467
TACTCGA49250.07.68118435
TTACGAC21400.07.67325329
TAGAACA47850.07.659209712
ACACGCG19850.07.59148076
Produced by FastQC (version 0.11.5)