FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l05_n02_E3W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l05_n02_E3W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27909047
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT26700.012.990292
GTAGAAC36100.010.89772411
GCGTGAC39150.09.6070351
GTACTCG53600.09.4111314
CTCTATA77350.09.05594245
GGAAAAT109350.08.8151481
TACTCGA57800.08.6875985
AGCCTAG26350.08.6248625
GGTAGAA48750.08.57687810
GGGAAAT56200.08.5358731
GTGACTC46950.08.489933
CACTCGT82050.08.33354935
AGTACTC62050.08.31113153
GGACTTA42450.08.2757154
GCCGAAT22600.08.2713111
AATCGAA51600.08.2477375
AAAATCC76850.08.1757343
GACTTAT44100.08.16966155
GAATCGA41350.08.1146424
TAGAACA49600.08.11431612
Produced by FastQC (version 0.11.5)