FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l05_n02_E3W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l05_n02_E3W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29875422
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT36800.014.1983192
GCGTGAC47700.011.7561951
GTGACTC51950.010.5771393
GTAGAAC42050.010.37190311
GTACTCG64900.010.1668264
AGTACTC73450.09.4432793
TACTCGA70100.09.2190055
GAATCGA43050.09.1543464
GCTCTAG63350.09.01383911
GGAAAAT121450.08.9749841
AATCGAA52850.08.8195315
TCACACG29250.08.6113415
GGGAAAT57850.08.4088421
GAATTGA70900.08.3535674
TGACTCT70600.08.2933724
ATTGACC50200.08.1542656
GGTAGGC27200.08.018147
CTCTATA71650.08.01568145
TAGAACA54300.07.991766512
TCAGGTA60450.07.92235147
Produced by FastQC (version 0.11.5)