FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l05_n02_E3W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l05_n02_E3W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27143229
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT26800.015.8832682
GCGTGAC37450.012.5087511
GTACTCG68300.010.4519974
GTGACTC44400.010.4484193
AGTACTC74550.09.9685643
GTAGAAC41250.09.86438511
TACTCGA73150.09.7274845
GCTCTAG51700.09.43592611
GGAAAAT113900.09.2341211
TCACACG25350.09.04894415
AATCGAA51300.08.6911165
AATTGAC43150.08.610555
AAAATCC83750.08.38953
GAATTGA63500.08.2630984
TGACTCT59800.08.2094864
TAGAACA49950.08.14752612
ATTGACC43900.08.1461356
GGCGAAT16150.08.0883121
GAATCGA47000.07.9537314
CTCTATA55050.07.89655745
Produced by FastQC (version 0.11.5)