FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l05_n02_E3W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l05_n02_E3W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29478393
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT31000.015.9825962
GCGTGAC42250.012.3665031
GTGACTC47700.010.9997983
GTACTCG67700.010.5774734
GCTCTAG56700.09.59485911
AGTACTC75450.09.4910683
GGAAAAT127350.09.3549691
AATCGAA48000.09.2412665
GTAGAAC42150.09.17354411
AAAATCC89400.09.1183913
TACTCGA78600.08.7374325
GAATTGA70150.08.6993154
CTCTATA63300.08.68211845
GAATCGA39250.08.4911314
AATTGAC52600.08.3902875
ATTGACC49500.08.3603016
TCACACG30600.08.304790515
TGACTCT68600.08.2393524
AAATCCT95100.07.86142064
GGGAAAT62250.07.85065651
Produced by FastQC (version 0.11.5)