FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l05_n02_E3W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l05_n02_E3W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22863554
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN310160.13565694992125896No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT22100.015.2862962
GCGTGAC30800.012.3573671
GTGACTC35600.09.8683923
GAATCGA31800.09.2063624
GTACTCG49400.09.1174814
GAATTGA56150.09.1043284
GTAGAAC30350.08.88867911
ACACGCG24650.08.8506286
TCACACG22250.08.79375315
AGTACTC54400.08.6934513
GGAAAAT93400.08.6081791
GCTCTAG44950.08.5022511
TACTCGA51050.08.46900755
ATTGACC35750.08.304576
CTCTATA50400.08.26738745
ACTCGTG51300.08.02565736
AATTGAC37650.08.0143225
AATCGAA39400.08.0012685
CACTCGT50950.07.99042535
TGACTCT48350.07.8250054
Produced by FastQC (version 0.11.5)