FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l05_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l05_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13982952
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG3250.033.89809815
CGTCTGA3650.030.18323716
CACGTCT4500.024.4804714
CACACGT5050.022.70644612
ACACGTC4900.022.4833513
GAGACCG4900.021.59385131
CAGTCAC4100.020.84277527
CCGAGAC5000.020.6969729
ACCGATC4450.020.23761734
AGACCGA5600.018.08884432
CGAGACC6350.017.36743230
AGCACAC6700.017.1145610
GGAATGC6750.016.32095723
TCGGAAG11500.016.253213
ATCGGAA11650.016.2358472
CCGATCT5650.015.93936235
AGCGGTT7050.015.62706311
CCAGTCA5900.015.24627726
CGGAAGA12600.015.1917324
GCACACG7600.014.79199511
Produced by FastQC (version 0.11.5)