FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l05_n01_E3W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l05_n01_E3W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24913200
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATC34450.027.19465838
ACGTCTG39500.025.50710515
TCGTATG35850.023.74740645
CCGTACG41500.023.65850833
CGTCTGA42950.023.56294816
CTCGTAT37900.022.7007144
ACCGTAC46900.021.50769832
CACCGTA47350.020.96672431
ATCGGAA54600.018.784272
TCGGAAG54650.018.7670463
CACGTCT55000.018.68581214
GTAATCT54650.017.67943839
CACACGT59450.017.13568112
TACGTAA56350.017.02432436
ACGTAAT56100.016.85944437
CAGTCAC62550.016.28642827
AATCTCG64450.015.1314141
TAATCTC65750.014.83217440
AGTCACC71800.014.56447428
ACACGTC74500.014.27778113
Produced by FastQC (version 0.11.5)