FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l05_n01_E3W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l05_n01_E3W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26662323
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG37000.031.70896733
AGTCACG42450.027.86616128
GTTTCGG43800.026.99176234
ACGTCTG47100.025.59356715
TTTCGGA46400.025.13957235
CGTCTGA50000.024.19910216
TTCGGAA49900.023.64721936
ACGTTTC51900.022.77579732
GTCACGT56850.020.97322329
CTCGTAT39600.020.53127944
ATCGGAA59950.020.1468852
TCGGAAT60100.019.10952837
TCGTATG40000.018.91817345
TCGGAAG64600.018.73153
CACGTCT71350.017.5244414
CGGAATC66250.017.16688738
CACGTTT71300.016.9549131
CACACGT74250.016.44599512
ACACGTC76500.016.07987613
CAGTCAC79250.015.74891127
Produced by FastQC (version 0.11.5)