FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l05_n01_E3W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l05_n01_E3W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27422074
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG45800.024.94414328
ACGTCTG47250.024.70329915
CGTCTGA51850.022.29480716
CACGTGG51700.021.81478531
TCGTATG42500.021.09110345
GTCACGT54700.020.81049729
CTCGTAT47500.019.91432444
GGCCTTA60450.019.07311836
ATCGGAA62000.018.6462572
GCCTTAT60750.018.53448337
CACGTCT65150.018.2255714
TCGGAAG63800.018.0849023
TATCTCG62000.016.78257641
CACACGT72000.016.39775812
ACGTGGC71200.016.2223632
CCTTATC72450.015.63546938
CAGTCAC77900.015.41563727
ACACGTC80250.015.32853913
CTTATCT75450.014.9247839
TCACGTG77700.014.77173930
Produced by FastQC (version 0.11.5)