FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l05_n01_E3W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l05_n01_E3W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23566756
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG31100.020.53617128
TCGTATG31750.018.86902845
CTCGTAT32300.017.71106344
ACGTCTG36700.017.40376715
CGTGAAA35700.017.34663233
CGTCTGA38050.016.9636116
AACGATC35850.016.52150338
ACGTGAA38350.016.20404232
CACGTGA40450.015.30430431
GAAACGA42050.015.20924536
TGAAACG42100.014.76310435
AAACGAT43800.014.39612837
GTCACGT45450.013.95788429
TCGGAAG50200.012.7691383
CACGTCT50650.012.69831914
ATCGGAA51350.012.395572
CACACGT56850.011.82755212
TATAGGC47900.011.73704127
CAGTCAC57000.011.4413527
ACACGTC67550.010.15380113
Produced by FastQC (version 0.11.5)