FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l05_n01_E3W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l05_n01_E3W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27909047
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG47450.026.6369515
AGTCACG47050.026.52733228
CGTCTGA50050.025.02853816
TCGTATG46800.024.06297745
ACGTCCG52600.023.32864432
CTCGTAT50700.022.78973644
GTCCGCA55550.022.05323234
GTCACGT62400.020.15308629
TCGGAAG63950.019.800723
CACGTCT65800.019.48059314
ATCGGAA65600.019.02842
CCGCACA66100.018.49948536
TCACGTC68550.018.3841530
CACACGT76750.016.96480212
CAGTCAC76450.016.6195627
ACACGTC85300.015.60706413
TCCGCAC83350.015.07599635
CGCACAT82950.014.82342237
CGGAAGA94100.014.0062274
CGTCCGC92900.013.93826833
Produced by FastQC (version 0.11.5)