FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l05_n01_E3W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l05_n01_E3W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29875422
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG41500.024.06112315
CGTCTGA45750.021.72762116
GTCCGCA50550.018.93320736
CTCGTAT42450.018.62304144
TCGTATG42200.018.46641545
CACGTCT60050.017.15179314
TCGGAAG61400.016.8137233
ATCGGAA63100.016.3607372
CACACGT68700.014.73024312
CAGTCAC71300.013.94077427
CGCATCT67200.013.77474439
TCCGCAT69300.013.71346637
TCACCCG75350.013.17127830
AGTCACC80550.013.01019928
ACCCGTC77400.012.65373732
ACACGTC85000.012.64628913
CCGTCCG85350.012.32161234
CGTCCGC91750.011.46212235
CCCGTCC88400.011.43795233
AGCACAC97550.011.3420610
Produced by FastQC (version 0.11.5)