FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l05_n01_E3W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l05_n01_E3W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27143229
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG64350.030.92998315
CGTCTGA68250.029.09665116
TCGGAAG80950.024.6723143
TCGTATG39950.024.5241545
CACGTCT83000.024.24945314
ATCGGAA83600.023.8633352
GTCAGAA83650.023.28702236
CTCGTAT47450.023.16363344
GTCACAT85650.023.08754329
CAGTCAC87200.022.61676427
CACACGT90700.022.19029612
AGTCACA89200.022.05972728
TGTCAGA89450.021.85240435
ACACGTC102650.019.93562313
TCACATG102750.019.27483730
ATGTCAG106150.018.71145634
AATCTCG80850.018.35806341
CACATGT109300.018.31040831
ACATGTC109950.018.08257932
AGCACAC114050.018.0612410
Produced by FastQC (version 0.11.5)