FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l05_n01_E3W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l05_n01_E3W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29478393
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTAT41450.028.90441137
CCGTATC43950.027.51832638
AGTTCCG44650.027.18520234
CGTATCT44950.026.85716239
CGTCTGA47800.026.1121616
ACGTCTG48400.025.88138815
TTCCGTA49400.024.57153136
CTCGTAT45000.023.77441244
TCGTATG44100.023.69768345
TCGGAAG65500.019.6755963
ATCGGAA66250.019.147312
CACGTCT67750.018.98632214
GTTCCGT67400.018.71084435
CAGTCAC70100.017.9967127
TATCTCG66000.017.57483741
CACACGT72700.017.56961312
GTCACAG71100.017.52840629
AGTCACA74650.016.59891128
ACAGTTC76850.016.05522332
TCACAGT78000.015.86880730
Produced by FastQC (version 0.11.5)