FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l05_n01_E3W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l05_n01_E3W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22863554
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG42350.028.14506115
CGTCTGA44300.026.65233616
TCGTATG40350.025.2858745
CTCGTAT41300.024.32264944
TCGGAAG56650.021.0022983
ATCGGAA56550.020.999712
CACAGTC55850.020.75896331
CACGTCT57900.020.70163314
CACACGT62150.019.57512312
CAGTCAC62350.019.07952727
GTCACAG61000.019.06689829
AGTCACA63700.018.14622728
AGTCAAC63600.018.0227534
TCACAGT66000.017.62895430
AATCTCG63550.017.01178441
ACACGTC73800.016.78976213
ACAGTCA69900.016.5891832
GTCAACA73900.015.90693535
GATCGGA79550.015.098341
CCAGTCA79650.014.99191626
Produced by FastQC (version 0.11.5)