FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l04_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l04_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15034845
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN522490.34751937914890374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGT4150.033.68840437
GAGTGTA4250.030.6645528
CACTCTT6450.028.942752
TAGATCT4800.028.5575533
AGTGTAG4500.028.46258529
GTGTAGA4800.027.62031630
GTAGATC5050.026.69883232
GTGTAGG5750.023.8438117
TGTAGAT5600.023.6749531
TGTAGGG6200.022.47902718
TCGTGTA6150.022.28539515
CTCGGTG6700.022.25943638
GTAGGGA6100.021.7431719
TTCCCTA4300.021.415818
TAGGGAA6400.021.07549720
TCGGTGG6900.020.63565339
AGATCTC7050.020.40028834
GATCTCG7050.020.08173435
TCCCTAC4550.018.7572679
ACCTAGG901.8879629E-417.47515915
Produced by FastQC (version 0.11.5)