FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l04_n02_D3W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l04_n02_D3W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22976749
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN271340.11809329509583798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT19800.013.7605412
GTACTCG48600.011.1644264
GTAGAAC31350.010.97205311
TACTCGA51950.010.3577885
GCGTGAC30100.010.1737071
AAAATCC71900.09.8649553
AGTACTC59300.09.6827553
GGAAAAT97400.09.24714851
GAATTGA57300.09.1156784
GTGACTC33200.09.0204253
GGGAAAT45950.08.9185181
AAATCCT76600.08.8469454
AATCGAA45600.08.8377735
GAATCGA35100.08.7875764
AATTGAC35300.08.4188725
TAGAACA41500.08.34280912
GGTAGAA42950.08.2704810
TCACACG21800.08.25358615
TCAGGTA44750.08.2400057
CTCGAAT20500.08.2378571
Produced by FastQC (version 0.11.5)