FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l04_n02_D3W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l04_n02_D3W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23395447
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN278200.11891202591683758No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATCG32850.013.5033773
TAATCGA37050.013.429414
GTAGAAC42450.012.23354811
AATCGAG60700.010.4221755
TAGAACA53800.09.90354612
GGTAGAA55050.09.4744610
GAATCGA39600.09.3808374
TCAGGTA58900.09.3523577
CAGGTAG57650.09.3204378
GGAAAAT120500.09.3054831
AAAATCC85900.09.2532243
GCTAATC53150.09.0663262
CCCGAAT14350.08.3161171
GAATTGA61200.08.1668474
AATCGAA46650.08.0594535
AGATCAG99000.08.0049824
AAATCCT92650.07.97042134
AGGTAGA69050.07.7813889
GGTAAGT19500.07.499974317
GTGTGAG62200.07.44885315
Produced by FastQC (version 0.11.5)