FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l04_n02_D3W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l04_n02_D3W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25656616
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT19950.011.5129312
GTACTCG49250.010.331694
GTAGAAC38500.010.16070311
TACTCGA51350.09.8214765
GGAAAAT103450.08.9674161
TCAGGTA48850.08.5612327
AGTACTC62750.08.3611613
AATCGAA42900.08.1872215
GGTAGAA47750.08.09831310
TAGAACA49050.08.02129512
GTGACTC34200.07.8351133
GCGTGAC34750.07.51629451
AAAATCC76550.07.4127563
AGATCAG90050.07.3507694
GAATCGA41700.07.3429754
CAGGTAG54200.07.34231428
AAATCCT83950.07.32168634
AAAAATC73900.07.3129982
GTCCGTT37900.07.182235735
TTAAGTC17900.07.17051742
Produced by FastQC (version 0.11.5)