FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l04_n02_D3W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l04_n02_D3W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41361514
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT31400.012.04762652
GTAGAAC67700.010.99168211
CTATAAG49900.09.3846094
TCAGGTA87700.09.1782267
GGAAAAT175650.09.1401031
GTACTCG78750.09.03420454
TATAAGG53250.08.9632045
TACTCGA80150.08.960545
TAGAACA85500.08.8612512
AAAATCC132600.08.7624253
GCGTGAC47300.08.4736121
GGTAGAA87250.08.451611510
GGACTTA59050.08.1591934
CAGGTAG97950.08.0335478
AATCGAA78100.07.89853535
GACTTAT63200.07.76580435
GCTATAA61600.07.71293933
AAAAATC126950.07.6980172
AGTACTC101300.07.66880663
GAATCGA66150.07.5897664
Produced by FastQC (version 0.11.5)