FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l04_n02_D3W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l04_n02_D3W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25000734
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN291580.11662857578501495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT22800.013.8263712
GTAGAAC40500.012.98941511
GTACTCG57150.011.345564
TACTCGA57900.011.1983045
GCGTGAC31750.010.9924231
TCAGGTA51500.010.7399637
AGTACTC67750.010.20321753
TAGAACA53200.09.97333712
GGTAGAA52850.09.95410610
CAGGTAG59100.09.3962748
GTGACTC36550.09.24084953
AAAATCC76400.09.1953723
GGAAAAT102950.09.0985551
AGATCAG86250.08.8750234
GAATTGA58700.08.3995444
GGTAGGC25800.08.3661657
TCACACG24100.08.30595915
AATCGAA48350.08.0554165
GCTCTAG48250.07.827818411
AATTGAC38900.07.81308465
Produced by FastQC (version 0.11.5)