FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l04_n02_D3W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l04_n02_D3W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25793366
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT26350.015.5524132
GCGTGAC36100.012.0997441
GTAGAAC37950.011.2558811
GTGACTC41750.010.6244543
GTACTCG60600.09.9190974
GGAAAAT107800.09.7957061
TACTCGA61950.09.7030945
AATCGAA50450.09.3712685
TATAAGG30650.09.3285375
GAATCGA38150.09.2058434
GCTCTAG50200.09.09138411
AAAATCC79700.09.0121673
GGTAGAA49450.09.00203610
CTAGCAC48300.08.89356214
CTATAAG31450.08.8763524
AGTACTC70950.08.8541863
TCAGGTA51500.08.8192647
TAGAACA47750.08.80454112
GAATTGA59700.08.3716714
AAATCCT86550.08.0635844
Produced by FastQC (version 0.11.5)