FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l04_n02_D3W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l04_n02_D3W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23717402
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN293910.12392166730571923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT23100.015.8884112
GTACTCG51350.011.7058774
GCGTGAC33050.011.6500451
TACTCGA51850.011.3758035
GTAGAAC31500.011.20514111
GTGACTC37800.010.185743
AGTACTC64400.09.7894983
GGAAAAT99550.09.1830671
CTAGCAC43950.08.90380314
TAGAACA42000.08.885712
GGCGAAT14500.08.85135651
GCTCTAG46350.08.82774711
GGGAAAT49550.08.8157861
GGTAGAA42600.08.70775610
TCAGGTA46050.08.4466797
GAATTGA59950.08.2240944
AAAATCC73150.07.97211843
TCTAGCA55650.07.799227713
TGACTCT52450.07.72609764
GAATCGA33900.07.70356044
Produced by FastQC (version 0.11.5)