FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l04_n02_D3W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l04_n02_D3W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29532913
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT29000.017.7031252
GCGTGAC40350.013.7832771
GTGACTC48700.010.9579373
GGAAAAT125700.09.8878731
CTAGCAC54450.09.87157814
TACTCGA64700.09.8473815
GTACTCG66800.09.8413314
GCTCTAG58250.09.49418711
AAAATCC94600.09.4494993
GAATTGA73350.09.3308434
GTAGAAC41850.09.29328411
AATCGAA53850.08.98858455
AGTACTC77200.08.9542753
TATAAGG31050.08.8457945
GAATCGA40550.08.6612624
TCACACG30850.08.52988315
AAATCCT100200.08.4707354
TTAAGTC17850.08.3256632
AATTGAC49450.08.2404485
TGACTCT67650.08.18681054
Produced by FastQC (version 0.11.5)