FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l04_n02_D3W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l04_n02_D3W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22079996
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN265670.12032157976840213No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT16950.015.4098532
GTAGAAC28750.010.86898511
GTACTCG45400.010.4136654
GCGTGAC26700.010.2882611
TACTCGA47450.09.8685975
AAAATCC71600.09.8117133
GTGACTC30350.09.2737343
GGAAAAT99100.09.1564021
TCACACG22650.08.83757215
AGTACTC57100.08.7937093
AAATCCT77200.08.7487124
TCAGGTA40950.08.4552837
GAATTGA53750.08.125754
GGTAGAA39450.08.09206510
GAATCGA32150.08.0529874
AATCCTT66150.07.99772175
AATTGAC34500.07.89577155
TAGAACA38800.07.822164512
CCCGAAT13250.07.8169151
GGGGAAT44200.07.7430981
Produced by FastQC (version 0.11.5)