FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l04_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l04_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15034845
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA3200.030.20476316
ACGTCTG3150.029.97051815
CACACGT3850.025.68730712
AGACCGA4850.025.53191232
GAGACCG5250.024.01451331
CCGAGAC5400.023.35086329
CACGTCT4250.022.741514
ACCGATC5700.021.72517434
ACACGTC4500.020.97817613
GGAATGC7500.019.49198723
AGCGGTT7950.018.65964711
CGAGACC7050.018.52954730
AGATCGG8150.018.2265931
CGGTTCA8800.017.36829613
CCGATCT7850.017.2091135
AGCACAC6100.016.94935410
CAGTCAC3850.016.9480827
AGGAATG8800.016.60679622
ATCGGAA11650.015.0684672
TCGGAAG11950.014.8819343
Produced by FastQC (version 0.11.5)