FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l04_n01_D3W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l04_n01_D3W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22976749
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG35900.026.18280415
CGTAATC33800.026.0576438
CGTCTGA38550.024.38305316
CCGTACG40500.023.07794433
ACCGTAC43450.021.71818532
TCGTATG35250.021.21793745
CTCGTAT38000.020.80906344
ATCGGAA48700.019.5786862
TCGGAAG49450.019.2360953
CACCGTA49250.018.97213431
CACGTCT52100.018.25604614
TACGTAA52350.017.59659436
GTAATCT52650.017.02907239
CACACGT56950.016.89847412
ACGTAAT53850.016.60482837
CAGTCAC59300.016.04005427
AATCTCG56900.015.55998841
ACACGTC66150.015.05820513
TAATCTC61550.014.49359840
AGTCACC68850.014.33976828
Produced by FastQC (version 0.11.5)