FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l04_n01_D3W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l04_n01_D3W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23395447
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG36000.031.78234133
AGTCACG40400.028.44448728
ACGTCTG43200.026.96306815
GTTTCGG45000.025.57662834
CGTCTGA47100.024.96922116
ACGTTTC46150.024.88947532
TTCGGAA45350.024.88332236
TTTCGGA48050.023.71881535
GTCACGT49550.023.25236729
ATCGGAA52400.022.4870072
TCGGAAT51550.021.84752737
TCGGAAG55300.021.5109863
CTCGTAT34550.020.93196544
CACACGT59000.020.04542412
TCGTATG33950.019.2445445
CACGTCT64000.018.76079214
CACGTTT62600.018.52424231
ACACGTC66100.018.33472413
CGGAATC62250.018.02157838
GATCGGA72450.016.6683851
Produced by FastQC (version 0.11.5)