FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l04_n01_D3W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l04_n01_D3W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25656616
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG42350.025.53929515
AGTCACG42350.025.06333528
CGTCTGA47850.022.74472216
CACGTGG47750.022.25784131
TCGTATG38900.020.32840745
GTCACGT52600.020.23579829
CTCGTAT43450.019.8590444
GGCCTTA54600.019.3062636
TCGGAAG57500.019.202213
ATCGGAA57850.019.1250172
CACGTCT57950.019.05094314
GCCTTAT54400.019.0049737
TATCTCG50350.018.47983741
CACACGT62850.017.70852712
ACGTGGC62950.016.92332632
TCACGTG65850.016.30558230
ACACGTC76000.015.53212313
CAGTCAC72000.015.48999227
CCTTATC67650.015.38420538
CTTATCT67100.015.34364239
Produced by FastQC (version 0.11.5)