FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l04_n01_D3W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l04_n01_D3W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41361514
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG49700.020.04485928
ACGTCTG53550.019.148215
TCGTATG50550.018.40652745
CGTGAAA53000.018.40099733
CGTCTGA59700.017.28868516
CTCGTAT56250.015.70039944
ACGTGAA65750.015.17509932
AACGATC61550.014.82255638
CACGTGA68750.014.67291331
TGAAACG67500.014.51536535
GTCACGT73200.013.83359229
CACGTCT80350.013.32025814
TCGGAAG77900.013.2497693
ATCGGAA75850.013.222592
AAACGAT74000.012.75379737
CACACGT86500.012.37294312
TCACGTG86500.011.91805330
GAAACGA83900.011.57084736
CAGTCAC97250.011.14443327
TATAGGC76550.010.42758427
Produced by FastQC (version 0.11.5)